Molecular Identification of Leishmania spp. DNA from Archived Giemsa-Stained Slides of Patients from Salta, Argentina
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چکیده
منابع مشابه
Identification of Leishmania Species Using PCR Assay on Giemsa-Stained Slides Prepared From Cutaneous Leishmaniasis Patients
BACKGROUND Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. METHODS In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. The slides were prepared from the patients with cutaneous leishmaniasi...
متن کاملidentification of leishmania species using pcr assay on giemsa-stained slides prepared from cutaneous leishmaniasis patients.
background: leishmaniasis is a group of diseases that are created by intracellular parasites of leishmania. cutaneous leishmaniasis is considered as one of the health problems in some provinces of iran. methods: in this study, a total of 178 giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. the slides were prepared from the patients with cutaneous leishmaniasi...
متن کاملDNA extraction and amplification of leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous Leishmaniasis by PCR.
DNA was isolated from 92 Giemsa-stained smears of lesions from suspected cases of cutaneous leishmaniasis and used for PCR-based diagnosis of Leishmania infection. Each smear had been examined under a light microscope at x 1,000 and scored for amastigote numbers. Although the smears had been stored for up to 4 years, all the microscopy-positive slides were also positive by PCR and four of the 1...
متن کاملDiagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP
Background: Direct identification of Leishmania species in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from Giemsa-stained smears and to characterize them by PCR technique. Methods: A total of 48 Giemsa-Stained slides from confirmed cases of leishmaniasis were examined under a ligh...
متن کاملmolecular epidemiological survey of cutaneous leishmaniasis in two highly endemic metropolises of iran, application of fta cards for dna extraction from giemsa-stained slides
results fifty four of 84 (64.2%) slides obtained from patients had positive results microscopically, while 79/84 (94%) of slides had positive results by fta card-nested-pcr. pcr and microscopy showed a sensitivity of 96.4% and 64.2% and specificity of 100% and 100%, respectively. interestingly, leishmania major as causative agent of zoonotic cl was identified in 100% and 90.7% of cl cases from ...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 2018
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.18-0223